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Analysis of ofloxacin using UV Spectrophotometer

Ofloxacin belongs to the class of Floroquinolones antibiotics. This class of antibiotic is used for the treatment of both gram positive and gram negative bacterial A Simple, easy, economical and quantitative analytical method has been applied for the determination of ofloxacin using active and pharmaceutical dosage form with 2 different brands including Oflox400mg tablets manufactured by Indus Pharma Batch # 1008 and Tarivid 200mg tablet manufactured by Sanofi Aventis Batch # WD057. The maximum absorbance was detected at wavelength 294 nm using distilled water as a solvent. The linearity of the method was analyzed, ranging from 3.125ppm to 25 ppm and is found to be satisfied. Beers law was observed in the concentration range of 3.125-25 ppm with correlation coefficents 0.988, 0.998, 0.920 for standard, Oflox and Tarivid respectively which meet the criteria mentioned in the ICH guide lines (Figure 2-4). The linear regression equation obtained by least square regression method were y = 0.073x + 0.105 for active, y = 0.111x + 0.199 for Oflox and y = 0.12x + 0.527 for Tarivid which also meet the criteria mentioned in the ICH guide lines, where y is the absorbance and x is the concentration of the pure drug solution.


Safila Naveed and Fouzia Hamid

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